Watch now. The Chaperone tells the true, previously untold story of a lone school teacher who fought off an entire motorcycle gang while chaperoning a middle school dance in a church basement in s Montreal, Canada. Told from the first person unscripted perspective of the school teacher and DJ who were there that night, The Chaperone recreates the whole scene using hand drawn animation, miniature sets, puppets, live action Kung Fu and explosions all done in stereoscopic 3D. With over 10, hand drawings many of which were colored in crayon by hand , an original blaxploitation score and featuring a cast of over people, The Chaperone is an unconventional approach to documentary shorts.
Hsp70 is a chaperone protein that participates in the folding of de novo synthesized proteins, protection of the hydrophobic regions of denaturated proteins, the regulation of apoptosis, the immune response, and several other cellular processes. Despite the large number of publications devoted to the functioning and structure of Hsp70, a reliable full-size 3D structure of this protein remains currently unavailable. Several probable full-size models of human Hsp70 have been constructed based on the structures of individual domains and their components from different organisms and using molecular modeling methodology. The stability of the obtained structures was studied using molecular dynamics. As a result of such an analysis, the most adequate model was selected. The model was built on the basis of Hsp70 elements from Bos Taurus and Caenorhabditis elegans. Using the method of steered molecular dynamics, the key salt bridges responsible for the interdomain interactions were identified: Arg Glu and Arg Glu Based on the performed molecular modeling, the scheme of the mechanism triggering ATP hydrolysis and leading to the separation of ATPase and the substrate-binding domains was proposed. Its function is to protect from aggregation the hydrophobic surfaces of proteins denaturated under stress and to promote their correct folding or direct them toward degradation.
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Make social videos in an instant: use custom templates to tell the right story for your business. The previously untold, true story of a lone teacher chaperoning a peaceful school dance in a s Montreal church basement But the bikers don't know one thing: Ralph has a curriculum to teach, and he'll ram it into their skull if he has to! Learn everything you need to know about gang warfare with The Chaperone: the short that toured the world! Seen at over 70 festivals, winner of 25 awards in the most cutthroat industry of them all: show business!
This structure shows that the hydroxyl of threonine is located close to the gamma-phosphate of ATP, in a po This structure shows that the hydroxyl of threonine is located close to the gamma-phosphate of ATP, in a position where it might be an intermediate phosphate acceptor in the hydrolysis reaction. We made two point mutations at residue of Hsc70, threonine to valine TV and threonine to glutamic acid TE. The kcat values for the mutant proteins were also increased. After crystallization, the structures of the TV and TE proteins were solved and refined with data to 2.